At the department of Optical Nanoscopy, a new junior research group for bioluminescence imaging is being established to image living cells by the light they produce. To enable bioluminescence imaging without external substrates, the genetically encodable bioluminescence system from bacteria will be used. The focus is on the generation of new bioluminescent probes with enhanced brightness and spectral properties and their application in biomedical research. The PhD project consists in the development of bioluminescent labeling strategies to image neurons and cardiomyocytes as well as a genetically encoded calcium sensor to observe cellular activity.